Assay for intracellular calcium using a codon-optimized aequorin.

نویسندگان

  • Wendy I Vernon
  • John A Printen
چکیده

catabolite gene activator protein (CAP) I. Saturation and alanine-scanning mutagenesis. J. Mol. Biol. 243:595-602. 10.Rhodius, V.A., D.M. West, C.L. Webster, S.J.W. Busby, and N.J. Savery. 1997. Transcription activation at class II CRP-dependent promoters: the role of different activating regions. Nucleic Acids Res. 25:326-332. 11.Savery, N.J., G.S. Lloyd, M. Kainz, T. Gaal, W. Ross, R.H. Ebright, R.L. Gourse, and S.J.W. Busby. 1998. Transcription activation at class II CRP-dependent promoters: identification of determinants in the C-terminal domain of the RNA polymerase α-subunit. EMBO J. 17:3439-3447. 12.Schultz, S.C., G.C. Shields, and T.A. Steitz. 1991. Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees. Science 253:1001-1007. 13.Weber, I.T. and T.A. Steitz. 1987. Structure of a complex of catabolite gene activator protein and cyclic AMP refined at 2.5 Å resolution. J. Mol. Biol. 198:311-326. 14.Zhang, X.P., A. Gunasekera, Y.W. Ebright, and R.H. Ebright. 1991. Derivatives of CAP having no solvent-accessible cysteine residues, or having a unique solvent-accessible cysteine residue at amino acid 2 of the helix-turn-helix motif. J. Biol. Struc. Dyn. 9:463-473. 15.Zhou, Y., X. Zhang, and R.H. Ebright. 1993. Identification of the activating region of catabolite gene activator protein (CAP): isolation and characterization of mutants of CAP specifically defective in transcription activation. Proc. Natl. Acad. Sci. USA 90:60816085.

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عنوان ژورنال:
  • BioTechniques

دوره 33 4  شماره 

صفحات  -

تاریخ انتشار 2002